Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA ME003 Tumor sup.-3 probemix - 100 reactions

SALSA MLPA ME003 Tumor sup.-3 probemix - 100 reactions

application: Tumour suppressor genes
region: Various Detailní informace

Cena s DPH € 1 147.08
Cena bez DPH € 948.00
 100 react
Dostupnost Skladem
Kód produktu ME003-100R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA ME003 Tumor sup.-3 probemix - 100 reactions

ME003-100R SALSA MLPA ME003 Tumour suppressor mix 3 probemix – 100 rxn

Discounts on the ME001, ME002, ME003, ME004, ME046 kits are available for specific research projects. Please enquire.

Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of tumour suppressor genes in a wide spectrum of human cancers. CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes.

This ME003-A1 MS-MLPA probemix contains 27 MS-MLPA probes which detect the methylation status of promotor regions of 18 different tumour suppressor genes. These tumour suppressor genes are frequently silenced by methylation in tumours, but are unmethylated in blood-derived DNA of healthy individuals. In addition, 13 reference probes are included which are not affected by HhaI digestion. Besides detecting aberrant methylation, all 40 probes present will give information on copy number changes in the analyzed sample. The MLPA reaction requires as little as 20 ng of human DNA and can be used on a variety of DNA samples, including those derived from paraffin-embedded tissues. Please note that each MS-MLPA reaction generates two samples that need analysis by capillary electrophoresis: one undigested sample for copy number detection and one digested sample for methylation detection. More information about MS-MLPA can be found on page 2 and in the MS-MLPA protocol.

The MS-MLPA probes in this ME003-A1 probemix detect sequences in promoter regions of tumor suppressor genes that are unmethylated in most blood-derived DNA samples. Upon digestion, the peak signal obtained in unmethylated samples will be very small or absent. In contrast, when tested on in vitro methylated human DNA, these probes do generate a  signal. We have no data showing that methylation detected by a  particular probe indeed influences the corresponding mRNA levels. Moreover, most MS-MLPA probes target only a single specific HhaI site of the CpG island. If methylation is absent for this particular CpG-site, it does not necessarily mean that the complete CpG island is unmethylated. For samples containing both tumor and normal cells, MLPA will indicate the average copy number of genes and the average methylation level.

This SALSA MS-MLPA kit can be used to detect aberrant methylation of one or more sequences of tumor suppressor genes. Methylation levels can be different for different tissues. Please use DNA derived from the same type of tissue and purified by the same method as reference sample. This SALSA MS-MLPA kit can also be used to detect deletions and duplications of one or more sequences of the genes targeted by the probes. Heterozygous deletions of probe recognition sequences should give a  35-50% reduced relative peak area of the amplification product of that probe. However, mutations and/or polymorphisms very close to the probe ligation site may also result in a reduced relative peak area. Therefore, apparent deletions detected by a single probe always require confirmation by other methods.

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