Úvod Reagencie Methylation analysis (MS-MLPA) SALSA MLPA ME011 MMR probemix - 25 reactions

SALSA MLPA ME011 MMR probemix - 25 reactions

SALSA MLPA ME011 MMR probemix - 25 reactions

application: Mismatch repair genes (MMR)
region: MLH1, MSH2, MSH6, MLH3, MSH3, PMS2, MGMT Detailní informace

Cena s DPH € 286.77
Cena bez DPH € 237.00
 25 react
Dostupnost Na dotaz
Kód produktu ME011-025R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA ME011 MMR probemix - 25 reactions

ME011-025R SALSA MLPA ME011 Mismatch Repair genes probemix – 25 rxn

Pro nejaktuálnější informace o produktu navštivte stránky výrobce ME011-25R SALSA MLPA ME011 Mismatch Repair genes probemix – 25 rxn

MRC-Holland has prepared a mixture of female genomic DNA from healthy individuals and a carefully titrated amount of plasmid that contains the target sequence recognised by several probes present in the selected MLPA probemixes. The use of SD027 in MLPA reactions performed with the selected MLPA probemixes will therefore show a duplication of several sequences. This SD027 can be ordered separately.

The Mismatch Repair (MMR) system is critical for the maintenance of genomic stability. Cells with MMR deficiency may lead to the accumulation of mutations resulting in the initiation of cancer. Several MMR genes are involved in hereditary nonpolyposis colon cancer (HNPCC). Mutations in the MLH1 and MSH2 genes have been found in about 90% of HNPCC cases. Mutations in other MMR genes are less frequent detected in HNPCC patients. In sporadic colon cancer hypermethylation is more frequent mechanism than point mutations for transcriptional silencing of the MLH1 gene. Methylation analysis of MLH1 by MS-MLPA can improve the selection of patients for genetic testing for Lynch syndrome (Perez-Carbonell L et al. 2010, J Mol Diagn. 12:498-504). MGMT promoter methylation is an important molecular marker in various tumours such as glioblastomas. MGMT plays a role in removing O(6)-alkylguanine, which is the major mutagenic and carcinogenic lesion induced by alkylating mutagens.

This ME011-B2 Mismatch Repair genes probemix has been developed to detect aberrant CpG islands methylation of seven MMR genes and includes 6  probes for MLH1 (3p22.1), 6 probes specific for the MGMT promoter region (10q26), 4 probes for MSH2 (2p21), 3 probes for MSH6 (2p16), 3  probes for PMS2 (7p22), 2 probes for MSH3 (5q14.1), and 1 probe for MLH3 (14q24.3).

This probemix includes 22 probes containing a HhaI recognition site, which yields information about the methylation status of a target sequence. In addition, 13 reference probes are present which are not influenced by the HhaI digestion. Besides the detection of aberrant methylation, all probes present will give information on copy number changes in the analysed sample. More information about MS-MLPA can be found on page 2.

This SALSA® MS-MLPA® probemix can be used to detect aberrant methylation of one or more sequences of the MMR genes in a DNA sample. Methylation levels can be different for different tissues. If possible, use identically treated test and reference samples (same tissue type and extraction method). This SALSA® MS-MLPA® probemix can also be used to detect deletions and duplications of one or more sequences of the MMR genes. Heterozygous deletions of probe recognition sequences should give a 35-50% reduced relative peak height of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a  reduction in relative peak height, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings. Finally, note that most defects in this gene are expected to be small (point) mutations which will not be detected by this SALSA® MS-MLPA® test. We have no information on what percentage of defects in these genes is caused by deletions/duplications of complete exons.

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