Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P088 Glioma-1 probemix - 50 reactions

SALSA MLPA P088 Glioma-1 probemix - 50 reactions

application: Oligodendroglioma
region: 1p, 19q, IDH1/2, CDKN2A/2B Detailní informace

Cena s DPH € 573.54
Cena bez DPH € 474.00
 50 react
Dostupnost Skladem
Kód produktu P088-050R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P088 Glioma-1 probemix - 50 reactions

P088-050R SALSA MLPA P088 Oligodendroglioma probemix – 50 rxn

Gliomas are a central nervous system neoplasms derived from glial cells and comprise astrocytoma, ependymoma, glioblastoma multiforme and oligodendrogliomas. Oligodendroglioma is the second most common malignant brain tumour in adults and frequently shows characteristic co-deletion of the chromosomal arms 1p and 19q.

This P088-C1 probemix can be used to detect the loss of chromosome arms 1p and 19q in DNA samples obtained from glioma patients, as well as copy number changes in CDKN2A and CDKN2B genes. Furthermore, this probemix contains mutation-specific probes for the most common point mutations of IDH1 and IDH2 genes in gliomas. In addition, 14 reference probes have been included in this probemix, detecting different autosomal chromosomal locations, which are relatively quiet in glioma.

SD021 Sample DNA Please note that the mutation-specific probes have only been tested on control plasmids and not on positive human DNA samples with the IDH1 (R132C and R132H) and IDH2 (R172M and R172K) point mutations! This SD021 sample DNA is provided with each probemix vial and can be used in data binning in the fragment analysis and as a  positive control for the mutation-specific probes (see vial with blue cap).

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more of the above mentioned chromosomal regions and genes, as well as to identify the presence of the aforementioned mutations in a DNA sample. Heterozygous deletions of probe recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism (e.g. SNP) in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, single probe deletions or duplications detected by MLPA should always be confirmed by other methods or by MLPA probemixes with higher resolution in the gene or chromosomal area of interest. Users should always verify the latest scientific literature when interpreting their findings.

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