Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P146 CRC GAIN probemix - 25 reactions

SALSA MLPA P146 CRC GAIN probemix - 25 reactions

SALSA MLPA P146 CRC GAIN probemix - 25 reactions

application: Colon cancer
region: Various Detailní informace

Cena s DPH € 294.03
Cena bez DPH € 243.00
 25 react
Dostupnost Skladem
Kód produktu P146-025R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P146 CRC GAIN probemix - 25 reactions

P146-025R SALSA MLPA P146 CRC GAIN probemix – 25 rxn

Colorectal cancer (CRC) is the third most common malignant neoplasm worldwide. Despite recent advances in surgical techniques and in adjuvant therapy, there has been only a modest improvement in survival for CRC patients with advanced tumours. Gains of chromosome arms 8q, 13q and 20q are common in CRC samples and they are suggested to play an important role in the progression of this disease. Other chromosomal aberrations are suggested to associate with the progression of adenomas to carcinomas are losses of 8p, 15q, 17p and 18q, which can be analysed with the P413-CRC Loss probemix.

This P146-CRC Gain probemix contains 44 probes for the following chromosomal regions: 8q12.1-q24.3 (13 probes), 13q12.11-q31.3 (17 probes), 20q11.21-q13.33 (14 probes). In addition, 12 reference probes have been included in this probemix, detecting autosomal chromosomal locations which are relatively quiet in colorectal tumours. However, it should be noticed that CRC karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes.

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned chromosomal regions in a DNA sample. Heterozygous deletions of recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism (e.g. SNP) in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, single probe deletions or duplications detected by MLPA should always be confirmed by other methods or by MLPA probemixes with higher resolution in the gene or chromosomal area of interest. Users should always verify the latest scientific literature when interpreting their findings.

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