Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P247 Chemokine-2 probemix - 100 reactions

SALSA MLPA P247 Chemokine-2 probemix - 100 reactions

application: Chemokines
region: CXCR4, CX3CR1, CCR5, CCR2, CD4, CD209 Detailní informace

Cena s DPH € 1 147.08
Cena bez DPH € 948.00
 100 react
Dostupnost Skladem
Kód produktu P247-100R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P247 Chemokine-2 probemix - 100 reactions

P247-025R SALSA MLPA P247 Chemokine-2 probemix – 100 rxn

description
Chemokines and their receptors control immune cell migration during infections and autoimmune responses. Polymorphisms and copy number changes in the chemokine genes can alter the expression of these genes in the inflammatory cells, which can affect the clinical phenotype of the disease. For example, the variation in genes underlying host immunity can lead to differences in susceptibility to HIV infection among humans.
Chemokines are generally subdivided into C-C chemokines with 2 adjacent cysteine residues and C-X-C chemokines, in which the cysteines are separated by 1 amino acid. Members of the former subfamily predominantly attract monocytes/macrophages, whereas the latter family consists of neutrophil chemotactic proteins.
This P247-A2 Chemokine mix-2 probemix can be used to detect copy number changes of the following six genes:

§ The CXCR4 gene (2 exons) spans ~4 kb of genomic DNA and is located on 2q21.3, 137 Mb from the p-telomere. The P247-A2 probemix contains one probe for each of the two exons.

§ The CX3CR1 gene (2 exons) spans ~17 kb of genomic DNA and is located on 3p22.2, 39 Mb from the p-telomere. The P247-A2 probemix contains two probes for each of the two exons.

§ The CCR5 gene (3 exons) spans ~6 kb of genomic DNA and is located on 3p21.31, 46 Mb from the p-telomere. The P247-A2 probemix contains one probe each for exons 1 and 2 and two probes for exon 3.

§ The CCR2 gene (3 exons) spans ~7 kb of genomic DNA and is located on 3p21.31, 46 Mb from p-telomere. The P247-A2 probemix contains two probes each for exons 1 and 3 and one probe for exon 2.

§ The CD4 gene (10 exons) spans ~32 kb of genomic DNA and is located on 12p13.31, 6.7 Mb from p-telomere. The P247-A2 probemix contains one probe for each of the exons with the exception of exon 9.

§ The CD209 (DC-SIGN) gene (6 exons) spans ~8 kb of genomic DNA and is located on 19p13.2, 7.7 Mb from p-telomere. The P247-A2 probemix contains one probe for exon 5 and two for exon 6.

In addition, the probemix includes probes that are specific for the CX3CR1 V249I and T280M mutations, the CCR2 V64I mutation and the CCR5-delta-32 mutation. These mutation-specific probes will only generate a signal when a mutation is present with the exception of the CCR5-delta-32 mutation which will generate a signal 32 nt shorter than normal (251 nt instead of the normal wildtype specific signal at 283 nt).

SD031 Sample DNA
Please note that the mutation-specific probes have only been tested on control plasmids and not on positive human DNA samples with the V249I, T280M, V64I and CCR5-delta-32 mutations! This SD031 sample DNA is provided with each probemix vial and can be used in data binning in the fragment analysis and as a positive control for the mutation-specific probes (see next page).

This SALSA® MLPA® probemix is designed to detect deletions/duplications of one or more sequences in the aforementioned genes and to detect the presence of the aforementioned mutations in a DNA sample. Heterozygous deletions of recognition sequences should give a 35-50% reduced relative peak height of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak height, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings. Finally, note that most defects in these genes are expected to be small mutations which will not be detected by this SALSA® MLPA® test. 

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