Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P252 NB mix 2 probemix - 34 reactions

SALSA MLPA P252 NB mix 2 probemix - 34 reactions

SALSA MLPA P252 NB mix 2 probemix - 34 reactions

application: Neuroblastoma
region: 2p24.1/MYCN, 2q33, 17p13/TP53, 17q Detailní informace

Cena s DPH € 382.36
Cena bez DPH € 316.00
 34 react
Dostupnost Skladem
Kód produktu P252-034R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P252 NB mix 2 probemix - 34 reactions

P252-025R SALSA MLPA P252 NB mix 2 probemix – 34 rxn

description
The P251/P252/P253 Neuroblastoma probemixes can be used to detect copy number changes of several chromosomal regions that frequently show copy number changes in neuroblastoma tumours. These three probemixes have replaced the older P161/P162 Neuroblastoma probemixes.

Neuroblastoma is a relatively common cancer of childhood that usually occurs sporadically (i.e. non-hereditary). Neuroblastoma is characterized by striking clinical heterogeneity, including subsets that show spontaneous tumour regression. The disease is lethal in over 40% of cases and is responsible for 15% of all cancer-related deaths in children. Several acquired genetic alterations such as amplification of the MYCN oncogene, deletions of chromosome bands 1p36 and 11q23 and unbalanced gains of 17q regions have been well-characterized and shown to be correlated with tumour behaviour, including response to treatment.

The P251 probemix contains probes for chromosomes 1, 3 and 11. The P252 probemix contains probes for chromosomes 2 (MYCN region) and 17 and the P253 probemix for chromosomes 4, 7, 9, 12 and 14. More information about these regions can be found on the following pages.

P252 competitor solution information
Samples with very high levels of MYCN amplification can result in low signals for other probes, making it difficult to analyse the latter. Therefore, all P252 probemixes are shipped together with a vial of the P252 competitor mix. This competitor solution contains oligonucleotides that can be included at the start of the MLPA reaction. Adding the competitors specifically reduces the signal of the eight MYCN region probes, making it possible to examine changes in other genes/chromosomal areas. When a sample shows a very high level of MYCN amplification the sample can be retested with the competitor solution:

Denature 4 µl DNA sample by heating 5 minutes at 98oC.
Add 1.5µl MLPA Buffer + 1.5 µl P252 probemix + 1µl of P252 Competitor solution.
Proceed with the MLPA protocol: 1 minute heating at 95oC, 16 hrs incubation at 60oC etc.

This SALSA® MLPA® probemix is designed to detect deletions/duplications of one or more genes/chromosomal areas in DNA sample. Heterozygous deletions of probe recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings.
 

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