Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P277 Telomere-10 probemix - 50 reactions

SALSA MLPA P277 Telomere-10 probemix - 50 reactions

application: Subtelomeric testing
region: 5q, 6q, 7q, 8q Detailní informace

Cena s DPH € 573.54
Cena bez DPH € 474.00
 50 react
Dostupnost Skladem
Kód produktu P277-050R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P277 Telomere-10 probemix - 50 reactions

P277-025R SALSA MLPA P277 Human Telomere-10 probemix – 50 rxn

description
This SALSA MLPA probemix can also be bought as part of the telomere follow-up set. The Telomere Follow-up Set covers all subtelomeres and contains 12 telomere follow-up probemixes: 465 telomere probes divided over 46 regions. The introduction price (valid in 2012) for 12 × 25 reactions is € 1200. Please refer to item no. "TELO" and description "Telomere Follow-up Set" on your order.

This P277-B1 probemix can be used to confirm and further characterise abnormalities detected by SALSA MLPA probemix P036 and/or SALSA MLPA probemixes P069 / P070 used for broad human subtelomere screening.

This SALSA® MLPA® P277-B1 Human Telomere-10 probemix contains:
14 probes in the terminal 6.8 Mb of chromosome arm 5q
11 probes in the terminal 4.5 Mb of chromosome arm 6q
11 probes in the terminal 6.8 Mb of chromosome arm 7q
11 probes in the terminal 4.6 Mb of chromosome arm 8q

SALSA MLPA probemixes P036 and P069/P070 each contain one MLPA probe for each subtelomeric region. Most of these probes detect a well-characterized gene close to the telomere. The probes in P036 and P069 / P070 are different, allowing results obtained with one probemix to be confirmed by the other. The majority of probes in P069 and P070 are identical. For the p telomeres of the acrocentric chromosomes 13, 14, 15, 21 and 22, it is not possible to design reliable probes. For these chromosomes, the P069 probemix only contains a probe for the q telomeric region. The P036 and P070 probemixes contain a second probe for these q arms, which target a DNA sequence close to the centromere.

We recommend using SALSA MLPA probemix P036 Human Telomere-3 and SALSA MLPA probemix P070 Human Telomere-5 for primary screening of the subtelomeres. Results obtained with these probemixes can be confirmed and further characterized with one of the specific follow-up probemixes for telomere analysis, such as this P277-B1 Human Telomere-10 probemix.

This SALSA® MLPA® probemix is designed to detect deletions/duplications of one or more sequences in the above mentioned chromosomal regions in a DNA sample. Heterozygous deletions of recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings.
 

Kontaktujte nás

BIOGEN PRAHA s.r.o.
Ke sv. Izidoru 2293/4A
140 00 PRAHA 4

Tel.: +420 241 401 693
Fax: +420 241 401 694
E-mail: biogen@biogen.cz

Novinky na e-mail