Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P294 Tumour-Loss probemix - 100 reactions

SALSA MLPA P294 Tumour-Loss probemix - 100 reactions

application: Tumour-Loss
region: various Detailní informace

Cena s DPH € 1 147.08
Cena bez DPH € 948.00
 100 react
Dostupnost Skladem
Kód produktu P294-100R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P294 Tumour-Loss probemix - 100 reactions

P294-025R SALSA MLPA P294 Tumour-Loss probemix – 100 rxn

description
The discount applies only for the complete kit and the probemix only, but not for the mixed kit.

The P294-B1 Tumour-Loss MLPA probemix contains 50 probes divided over 15 different chromosomal regions that are frequently deleted in tumour samples. At least two probes are present for each gene or region. Included are the following genes or regions: 1p36, 13q14 (RB1), AMER1, APC, BRCA1/2, CAMTA1, CDKN2A/B, CHD5, FKBP8, NF1, PTCH1, PTEN, SMAD4, SMARCB1, STK11, TP53, TSC1/2, VHL, and WT1 that are deleted in various types of tumours. In addition, 12 reference probes have been included in this probemix in chromosomal areas, which are relatively quiet in most of tumour types. However, it should be noticed that tumour karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes.

Similarly, the P175-A2 Tumour-Gain probemix contains 50 different probes for genes like ERBB2/TOP2A, MYC, CCND1, EGFR and MET that are frequently amplified in tumour cells.

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned genes and chromosomal regions in a DNA sample. Heterozygous deletions of autosomal recognition sequences should give a 35-50% reduced relative peak height of the amplification product of that probe. Deletions of a probe’s recognition sequence on the X-chromosome will lead to a complete absence of the corresponding probe amplification product in males, whereas female heterozygotes are recognisable by a 35-50% reduction in relative peak height. The probe signals of probes detecting amplified regions also depends on the percentage of tumour cells in the sample. The MLPA results will indicate the average copy number change of the probe target sequences in the cells in the sample. High percentage normal cells in the tumour samples will result in a smaller change in probe signal. Samples may also contain a mixture of different populations of tumour cells, which will complicate analysis.

Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak height, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings. We have no information on what percentage of defects in these genes is caused by deletions/duplications of complete exons. Finally, note that most defects in this gene are expected to be small (point) mutations which will not be detected by this SALSA® MLPA® test.

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