Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P323 CDK4 HMGA2 MDM2 probemix - 100 reactions

SALSA MLPA P323 CDK4 HMGA2 MDM2 probemix - 100 reactions

application: Sarcoma and other tumour types
region: chromosome 12, MDM2, CDK4 and HMGA2 genes Detailní informace

Cena s DPH € 1 147.08
Cena bez DPH € 948.00
 100 react
Dostupnost Skladem
Kód produktu P323-100R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P323 CDK4 HMGA2 MDM2 probemix - 100 reactions

P323-050R SALSA MLPA P323 CDK4-HMGA2-MDM2 probemix – 100 rxn

Copy number changes of chromosome 12 and amplified segments of 12q arm are frequently observed in several types of sarcomas and also in other tumours. These amplifications can be used in defining genetically distinct subsets of tumours with differential diagnosis and/or prognosis.

The P323 CDK4-HMGA-MDM2 probemix can be used to detect copy number changes of chromosome 12 and to detect amplifications of the MDM2, CDK4 and HMGA2 genes.

Please note that this SALSA MLPA kit is intended for research purposes and has not been validated yet on a sufficient number of patient samples.

In liposarcomas, MDM2 and HMGA2 genes are consistently amplified in well-differentiated (WDLPS) and dedifferentiated (DDLPS) types of liposarcomas,
which can be used clinically in distinguishing them from benign lipomas (Italiano A. et al. 2008. Int J. Cancer). Moreover, DDLPS and WDLPS patients with MDM2-HMGA2 amplification have favourable prognosis compared to patients with both HMGA2-MDM2 and CDK4 amplifications (Italiano A. et al. 2009, Clin Cancer Res). In osteosarcoma (OS), MDM2-CDK4 amplification can be used in differential diagnostics, since MDM2-CDK4 amplification seems to be most prevalent in parosteal OS (Mejia-Guerrero S. et al. 2010, Genes Chromosomes Cancer). Amplifications of 12q13-14 (including CDK4, HMGA2, MDM2, TSPAN31 and GLI1 genes) are common in leiomyosarcoma and alveaolar/embryonal/sclerosing rhabdomyosarcoma and 12q13-q14 amplifications in alveolar rhabdomyosarcoma correlate with poor survival (Barr F. et al. 2009, Genes Chromosomes Cancer). HMGA2 amplifications are characteristic for pituitary adenomas, and especially to prolactiomas (Finelli P. et al. 2002, Cancer Res). HMGA2 amplifications are also observed in adenomas and carcinomas of salivary glands (Persson F. et al. 2009, Genes Chromosomes Cancer).

This probemix contains 34 probes detecting copy number changes of chromosome 12, including 2 probes for CDK4, 5 probes for HMGA2 and 4 probes for the MDM2 gene. In addition, 12 reference probes are included in this probe mix detecting 12 different autosomal chromosome locations, which are relatively quiet in sarcomas and tumours in general. However, it should be noticed, that tumour karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes.

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned genes and chromosomal regions in a DNA sample. Heterozygous deletions of recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, apparent deletions detected by a single probe always require confirmation by other methods. Moreover, users should always verify the latest scientific literature when interpreting their findings.

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