Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P380 Wilms' tumour probemix - 50 reactions

SALSA MLPA P380 Wilms' tumour probemix - 50 reactions

application: Wilms' tumour
region: various. Detailní informace

Cena s DPH € 573.54
Cena bez DPH € 474.00
 50 react
Dostupnost Skladem
Kód produktu P380-050R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P380 Wilms' tumour probemix - 50 reactions

P380-025R SALSA MLPA P380 Wilms' tumour probemix – 50 rxn

Wilms’ tumour (WT) is the most common paediatric renal tumour. Most of the cases occur sporadically in otherwise normal children; however, a minority is associated with other developmental abnormalities such as WARG, Denys-Drash, Beckwith-Wiedemann or Frasier syndromes. Survival of children with Wilms’ tumour has increased to ~90 %, but survival of patients with relapse is still disappointingly low (~ 50%). Copy number changes of certain chromosomal regions and genes have been found to be highly significant for prognosis. Tools to classify these high-risk Wilms’ tumour patients are needed to intensify the treatment and, in addition, to reduce the treatment burden of the low-risk patients.

The P380 Wilms’ tumour probemix can be used to determine the copy number status of chromosomal arms 1p (7 probes), 1q (5 probes), 16p (3 probes) and 16q (6 probes). This probemix can further be used to determine the copy number of WT1 on 11p13 (3 probes), FBXW7 on 4q31.3 (3 probes), AMER1 (previously known as FAM123B) on Xq11.1 (3 probes), MYCN on 2p24.3 (3 probes) and TP53 on 17p13.1 (3 probes). Additionally, flanking probes (at 2p, 2q, 4q, 11p, 17p, Xp and Xq) are included to facilitate the determination of the extent of a deletion or duplication. In addition, 9 reference probes are included in this probemix, detecting 9 different autosomal chromosomal locations which are relatively stable in Wilms’ tumour samples.

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned genes and chromosomal regions in a DNA sample. Heterozygous deletions of autosomal recognition sequences should give a 35-50% reduced relative peak height of the amplification product of that probe. Deletions of a probe’s recognition sequence on the X-chromosome will lead to a complete absence of the corresponding probe amplification product in males, whereas female heterozygotes are recognisable by a 35-50% reduction in relative peak height. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak height, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, single probe deletions and duplications detected by MLPA should always be confirmed by other methods. Not all deletions and duplications detected by MLPA will be pathogenic; users should always verify the latest scientific literature when interpreting their findings.

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