SALSA MLPA P383 T-ALL probemix - 50 reactions

application: T-ALL

region: various Detailní informace

Cena s DPH € 573.54
Cena bez DPH € 474.00
 50 react
Dostupnost Skladem
Kód produktu P383-050R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland (nové okno)

Detailní popis SALSA MLPA P383 T-ALL probemix - 50 reactions

P383-025R SALSA MLPA P383 T-ALL probemix – 50 rxn

T-lineage acute lymphoblastic leukaemia (T-ALL) is a clonal malignant disorder of immature T-cells, which accounts for about 15% of paediatric and 25% of adult ALL cases. Although prognosis has improved due to effective treatment protocols, still around 25% of paediatric and 50-70% of adult patients experience relapse. T-ALL is genetically heterogeneous, and comprises multiple distinct subtypes defined by chromosomal rearrangements involving usually one of the T-cell receptor loci, but also non-TCR-driven translocations are detected. In addition, genome-wide characterisation has identified in recent years several novel amplifications, deletions and point mutations in T-ALL samples that are of interest to better define T-ALL subgroups.

This probemix contains 56 probes for 13 different chromosomal regions, which are suggested to be of diagnostic and/or prognostic importance in T-ALL. These chromosomal areas and genes are: STIL-TAL1 (1p33), LEF1 (4q25), CASP8AP2 (6q15), MYB (6q23.3), EZH2 (7q36.1) CDKN2A/B+MTAP+MLLT3 (9p21.3), NUP214-ABL1 (9q34.1), PTEN (10q23.31), LMO1 (11p15.4), LMO2 (11p13), NF1+SUZ12 (17q11.2), PTPN2 (18p11.21) and PHF6 (Xq26.2).

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned genes and chromosomal regions. Heterozygous deletions of autosomal recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Deletions of a probe’s recognition sequence on the X-chromosome will lead to a complete absence of the corresponding probe amplification product in males, whereas female heterozygotes are recognisable by a 35-50% reduction in relative peak area. Note that a mutation or polymorphism (e.g. SNP) in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals may be more sensitive to sample purity and small changes in experimental conditions. Therefore, single probe deletions / duplications and amplifications detected by MLPA should always be confirmed by other methods or by MLPA probemixes with higher resolution in the gene or chromosomal area of interest. Moreover, users should always verify the latest scientific literature when interpreting their findings.

SALSA® MLPA® probemixes and reagents are sold by MRC-Holland for research purposes and to demonstrate the possibilities of the MLPA technique. They are not CE/FDA certified for use in diagnostic procedures. Purchase of the SALSA® MLPA® test probemixes and reagents includes a limited license to use these products for research purposes.

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