Úvod Laboratorní plasty Zkumavky Microcentrifuge Tube 1.5 ml Microcentrifuge Tube SALSA MLPA P413 CRC LOSS probemix - 100 reactions

SALSA MLPA P413 CRC LOSS probemix - 100 reactions

Colorectal adenoma/carcinoma.

region: 8p, 15q, 17p, 18q Detailní informace

Cena s DPH € 1 147.08
Cena bez DPH € 948.00
 100 react
Dostupnost Skladem
Kód produktu P413-100R

Nejnovější informace o produktu naleznete exklusivně na stránkách výrobce MRC-Holland www.mlpa.com (nové okno)

Detailní popis SALSA MLPA P413 CRC LOSS probemix - 100 reactions

P413-025R SALSA MLPA P413 CRC LOSS probemix – 100 rxn

description
Colorectal cancer (CRC) is the third most common malignant neoplasm worldwide, and the incidence rate is still rising. Despite recent advances in surgical techniques and in adjuvant therapy, there has been only a modest improvement in survival for CRC patients with advanced tumours. Losses of chromosome arms 8p, 15q, 17p and/or 18q are common in CRC samples and they have be suggested to play an important role in the progression of this disease. It is suggested that loss of these regions contributes to the progression of adenomas to carcinomas and this P413-B1 CRC loss probemix contains probes for all these four regions.
This probemix contains 37 probes for the following chromosomal regions: 8p21-8p23.3 (10 probes), 15q11.2-q21.3 (9 probes), 17p11.2-p13.3 (9 probes) and 18q12.1-18q21.33 (9 probes). In addition, 13 reference probes have been included in this probemix, detecting autosomal chromosomal locations which are relatively quiet in colorectal tumours. However, it should be noticed that CRC karyotypes can harbour multiple numerical and structural aberrations, which can complicate interpretation of these reference probes.

This SALSA® MLPA® probemix is designed to detect copy number changes of one or more sequences in the above mentioned genes and chromosomal regions in a DNA sample. Heterozygous deletions of recognition sequences should give a 35-50% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplications detected by MLPA should always be confirmed by other methods or by MLPA probemixes with higher resolution in the gene or chromosomal area of interest. Users should always verify the latest scientific literature when interpreting their findings. Finally, note that most defects in these genes are expected to be small (point) mutations which will not be detected by this SALSA® MLPA® probemix.

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